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Tau antibody

Anti-Tau antibody used in IHC (Frozen sections) (IHC-Fr). GTX112981
Anti-Tau antibody used in IHC (Paraffin sections) (IHC-P). GTX112981
Anti-Tau antibody used in IHC (Paraffin sections) (IHC-P). GTX112981
Anti-Tau antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112981
Anti-Tau antibody used in Western Blot (WB). GTX112981
Anti-Tau antibody used in Western Blot (WB). GTX112981

Cat. No. GTX112981

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Tau products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

79 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , IMR32 , SK-N-AS

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.12 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Tau. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10730753

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

microtubule associated protein tau , DDPAC , FTDP-17 , MAPTL , MSTD , MTBT1 , MTBT2 , PPND , PPP1R103 , TAU

Cellular Localization

Cytoplasm , cytosol , Cell membrane; Peripheral membrane protein; Cytoplasmic side , cytoskeleton , Cell projection , axon

Background

This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Tau antibody used in IHC (Frozen sections) (IHC-Fr). GTX112981

GTX112981 IHC-Fr Image

Tau antibody detects Tau protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: Tau protein stained by Tau antibody (GTX112981) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-Tau antibody used in IHC (Paraffin sections) (IHC-P). GTX112981

GTX112981 IHC-P Image

Tau antibody detects Tau protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: Tau stained by Tau antibody (GTX112981) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Tau antibody used in IHC (Paraffin sections) (IHC-P). GTX112981

GTX112981 IHC-P Image

Tau antibody detects Tau protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
Tau stained by Tau antibody (GTX112981) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Tau antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112981

GTX112981 ICC/IF Image

Tau antibody detects Tau protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Tau stained by Tau antibody (GTX112981) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Tau antibody used in Western Blot (WB). GTX112981

GTX112981 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Tau antibody (GTX112981) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Tau antibody used in Western Blot (WB). GTX112981

GTX112981 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Tau antibody (GTX112981) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Espinosa-Jim?nez T et al. Cell Biosci 2023; 13 (1) : 52 A novel rhein-huprine hybrid ameliorates disease-modifying properties in preclinical mice model of Alzheimer's disease exacerbated with high fat diet.
Application : WB
Reactivity : Mouse
Chang YL et al. BMC Biol 2023; 21 (1) : 293 The HSP40 family chaperone isoform DNAJB6b prevents neuronal cells from tau aggregation.
Chen TF et al. Part Fibre Toxicol 2022; 19 (1) : 44 White matter pathology in alzheimer's transgenic mice with chronic exposure to low-level ambient fine particulate matter.
Parul Katiyar et al. Curr Res Toxicol 2022; 3 : 100072 Triclosan-induced neuroinflammation develops caspase-independent and TNF-α signaling pathway associated necroptosis in Neuro-2a cells.
Application : WB
Reactivity : Mouse
Sheng-Han Lee et al. PLoS One 2021; 16 (8) : e0254587 Three month inhalation exposure to low-level PM2.5 induced brain toxicity in an Alzheimer's disease mouse model.
Application : WB
Reactivity : Mouse
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169