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Transaldolase antibody [N2C3]

Anti-Transaldolase antibody [N2C3] used in Western Blot (WB). GTX114956
Anti-Transaldolase antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114956

Cat. No. GTX114956

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Transaldolase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

U87-MG , MCF-7

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Transaldolase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11164323

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transaldolase 1 , TAL , TAL-H , TALDOR , TALH

Cellular Localization

Cytoplasm

Background

Transaldolase 1 is a key enzyme of the nonoxidative pentose phosphate pathway providing ribose-5-phosphate for nucleic acid synthesis and NADPH for lipid biosynthesis. This pathway can also maintain glutathione at a reduced state and thus protect sulfhydryl groups and cellular integrity from oxygen radicals. The functional gene of transaldolase 1 is located on chromosome 11 and a pseudogene is identified on chromosome 1 but there are conflicting map locations. The second and third exon of this gene were developed by insertion of a retrotransposable element. This gene is thought to be involved in multiple sclerosis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Transaldolase antibody [N2C3] used in Western Blot (WB). GTX114956

GTX114956 WB Image

Sample (30 ug of whole cell lysate)
A: U87-MG
B: MCF-7
10% SDS PAGE
GTX114956 diluted at 1:2000

Anti-Transaldolase antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114956

GTX114956 ICC/IF Image

Transaldolase antibody [N2C3] detects Transaldolase protein at cytoplasm by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Transaldolase stained by Transaldolase antibody [N2C3] (GTX114956) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169