Transgelin antibody detects Transgelin protein at cytosol on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
Transgelin antibody (GTX113561) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Western blot analysis of Transgelin (GTX113561, upper panel) and beta-actin (lower panel) 
Sample (35 μg of whole cell lysate) 
A: A-10 cell
B: A-10 cell treated with MG132
GTX113561 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Transgelin antibody detects Transgelin protein at cytosol on mouse esophagus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
Transgelin antibody (GTX113561) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Transgelin antibody detects Transgelin protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Transgelin protein stained by Transgelin antibody (GTX113561) diluted at 1:100.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Hoechst 33342 staining.

Sample (50 μg of whole cell lysate)
A: mouse liver
12% SDS PAGE
GTX113561 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Transgelin antibody detects Transgelin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse smooth muscle.
Transgelin stained by Transgelin antibody (GTX113561) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Transgelin antibody (GTX113561) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Transgelin antibody (GTX113561) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The data was published in the journal Sci Rep in 2016. PMID: 27808256

The data was published in the journal Nat Commun in 2014. PMID: 24583950

The data was published in the journal Nat Commun in 2014. PMID: 24583950