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Trident Membrane Protein Extraction Kit

Trident Membrane Protein Extraction Kit. GTX16373
Trident Membrane Protein Extraction Kit. GTX16373

Cat. No. GTX16373

Reference 5

Application

 WB, IP, ELISA
Package
20 test ($319),
5 test ($159)
See all Extraction buffer products

APPLICATION

Application Note

1. Read the entire protocol carefully.
2. Before experiment, thaw buffer A or/and buffer B completely, invert the bottles a few times and keep them on ice. Place the filter cartridges in collection tubs, and pre-chill on ice. Pre-chill PBS on ice.
3. For IP, dissolve the extracted plasma membrane in PBS containing 0.5% Triton-X 100. Then do IP assay.

PROPERTIES

Storage

Store Buffer A and Buffer B at -20ºC, and the rest of the kit at room temperature.

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. Spin column-based protein extraction and cell fractionation technologies were developed by Invent Biotechnologies, Inc.

TARGET

Background

Trident Membrane protein extraction kit is for rapid extraction of native total membrane proteins from cultured mammalian cells or tissues.

  • Simple and user friendly
  • Wide range of starting cells (1 - 50 millions / sample)
  • Detergent and EDTA free
  • No need for Dounce homogenizer or tissue blender
  • The procedure can be completed in less than 45 min.
  • High yield

DATA IMAGES

Trident Membrane Protein Extraction Kit. GTX16373

GTX16373 WB Image

HepG2 cells were lysed and extracted by GTX16373 Plasma Membrane Protein Extraction kit.
When following the protocol, cell extracts would be isolated into cytosolic and total membrane.
The fractions were then assayed by Western Blot analysis, membrane was blotted with a plasma membrane protein- Na/K ATPase antibody (subcellular marker), a nucleolar small nuclear ribonucleoprotein- Fibrillarin antibody (GTX113684, subcellular marker) and beta Catenin antibody (GTX101435, internal control).
Abbreviations:
WCE : whole cell extract
CE : cytosolic extract
ME: total membrane extract
NE: nuclear extract

Trident Membrane Protein Extraction Kit. GTX16373

GTX16373 Image

REFERENCE

Application Reference

Aoyama S et al. Int J Mol Med 2022; 49 (4) A novel chimeric antigen receptor (CAR) system using an?exogenous protease, in which activation of T?cells is controlled by expression patterns of cell?surface proteins on target cells.
Utsumi T et al. Sci Rep 2021; 11 (1) : 19233 ANKRD22 is an N-myristoylated hairpin-like monotopic membrane protein specifically localized to lipid droplets.
Wu SH et al. J Biomed Sci 2021; 28 (1) : 19 The ESCRT-III molecules regulate the apical targeting of bile salt export pump.
Utsumi T et al. PLoS One 2018; 13 (11) : e0206355 Identification and characterization of protein N-myristoylation occurring on four human mitochondrial proteins, SAMM50, TOMM40, MIC19, and MIC25.
Yamamoto Y et al. Biochem Biophys Res Commun 2018; 495 (1) : 1292-1299 intracellular cholesterol level regulates sensitivity of glioblastoma cells against *-induced cell death by modulation of caspase-8 activation via death receptor 5-accumulation and activation in the plasma membrane lipid raft.
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Package List Price ($)
$ 319
$ 159