293T cells' cytoplasmic and nuclear proteins were extracted by 8-min. Cytoplasmic & Nuclear Protein Extraction Kit (Cat.# GTX16374).Courtesy of a GeneTex collaborating partner
A. SDS-PAGE gel shows the profiles of extracted cytoplasmic proteins and nuclear proteins.
B. The proteins from the gel were transferred to nitrocellulose membrane and probed with Histone deacetylase 1 (HDAC1) antibody (nuclear marker) and Actin antibody (cytoplasmic marker).