Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Tryptophanyl tRNA synthetase antibody [HL2186] (GTX638190) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Tryptophanyl tRNA synthetase antibody [HL2186] (GTX638190) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Tryptophanyl tRNA synthetase antibody [HL2186] detects Tryptophanyl tRNA synthetase protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: Tryptophanyl tRNA synthetase stained by Tryptophanyl tRNA synthetase antibody [HL2186] (GTX638190) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Tryptophanyl tRNA synthetase antibody [HL2186] (GTX638190) diluted at 1:1000. The HRP-conjated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Tryptophanyl tRNA synthetase antibody [HL2186] detects Tryptophanyl tRNA synthetase protein at cell membrane and secreted by immunohistochemical analysis.
Sample: Paraffin-embedded mouse adrenal gland.
Tryptophanyl tRNA synthetase stained by Tryptophanyl tRNA synthetase antibody [HL2186] (GTX638190) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Tryptophanyl tRNA synthetase antibody [HL2186] detects Tryptophanyl tRNA synthetase protein at cell membrane and secreted by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
Tryptophanyl tRNA synthetase stained by Tryptophanyl tRNA synthetase antibody [HL2186] (GTX638190) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Tryptophanyl tRNA synthetase antibody [HL2186] (GTX638190) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Tryptophanyl tRNA synthetase antibody [HL2186] (GTX638190) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.