Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with U2AF35 antibody (GTX106854) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using U2AF35(GTX106854) antibody at 1:200 dilution.

Immunoprecipitation of U2AF35 protein from 293T whole cell extracts using 5 μg of U2AF35 antibody (GTX106854).
Western blot analysis was performed using U2AF35 antibody (GTX106854).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

U2AF35 antibody detects U2AF35 protein at nucleus on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
U2AF35 antibody (GTX106854) diluted at 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min