UBE2C antibody [N1C3] detects UBE2C protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: UBE2C protein stained by UBE2C antibody [N1C3] (GTX100599) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Sample (30 ug of whole cell lysate)

A:NIH-3T3
12% SDS PAGE

GTX100599 diluted at 1:1000

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with UBE2C antibody [N1C3] (GTX100599) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Western Blot analysis of UBE2C expression in transfected 293T cell line by UBE2C polyclonal antibody.
A: Non-transfected lysate.
B: UBE2C transfected lysate.
12% SDS PAGE
GTX100599 diluted at 1:500