Wild-type (WT) and UBE2S knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with UBE2S antibody [N1C2] (GTX115862) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30 ug of whole cell lysate)
A: HCT116
12% SDS PAGE
GTX115862 diluted at 1:1000