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UCP1 antibody

Anti-UCP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX131207
Anti-UCP1 antibody used in Western Blot (WB). GTX131207

Cat. No. GTX131207

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Mouse
Package
100 μl ($399),
25 μl ($169)
See all UCP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

Mouse brown adipose

Predict Reactivity

Human, Rat, Rabbit, Sheep, Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.37 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human UCP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886443

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

uncoupling protein 1 , SLC25A7 , UCP

Cellular Localization

Mitochondrion inner membrane; Multi-pass membrane protein

Background

Mitochondrial uncoupling proteins (UCP) are members of the family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed only in brown adipose tissue, a specialized tissue which functions to produce heat. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-UCP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX131207

GTX131207 IHC-P Image

UCP1 antibody detects UCP1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brown adipocyte.
UCP1 stained by UCP1 antibody (GTX131207) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-UCP1 antibody used in Western Blot (WB). GTX131207

GTX131207 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with UCP1 antibody (GTX131207) diluted at 1:1000.

REFERENCE

Application Reference

Petrov PD et al. Am J Physiol Endocrinol Metab 2015; 308 (2) : E172-83 Improved metabolic regulation is associated with retinoblastoma protein gene haploinsufficiency in mice.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169