UHRF1 antibody immunoprecipitates UHRF1 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of UHRF1 protein by 2 μg of UHRF1 antibody (GTX113963) 5% SDS-PAGE The immunoprecipitated UHRF1 protein was detected by UHRF1 antibody (GTX113963) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Non-transfected (–) and transfected (+) HeLa whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with UHRF1 antibody (GTX113963) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with UHRF1 antibody (GTX113963) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

UHRF1 antibody detects UHRF1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: UHRF1 stained by UHRF1 antibody (GTX113963) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

UHRF1 antibody detects UHRF1 protein at on HBL435 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HBL435 xenograft.
UHRF1 antibody (GTX113963) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using UHRF1(GTX113963) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Cross-linked ChIP was performed with HCT116 chromatin extract and 5 μg of either control rabbit IgG or anti-UHRF1 antibody. The precipitated DNA was detected by PCR with primer set targeting to PPARG promoter.

Immunoprecipitation of UHRF1 protein from HeLa whole cell extract using 5 μg of UHRF1 antibody (GTX113963).
Western blot analysis was performed using UHRF1 antibody (GTX113963).
EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01).

The data was published in the journal Nucleic Acids Res in 2015. PMID: 25765655

The data was published in the journal Nucleic Acids Res in 2015. PMID: 25765655

The data was published in the journal Nucleic Acids Res in 2015. PMID: 25765655