Various whole cell extracts (60 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ULK1 antibody (GTX132669) diluted at 1:1000.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ULK1 antibody (GTX132669) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.