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UQCRFS1 antibody [N1C3]

Anti-UQCRFS1 antibody [N1C3] used in Western Blot (WB). GTX102150
Anti-UQCRFS1 antibody [N1C3] used in Western Blot (WB). GTX102150
Anti-UQCRFS1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102150
Anti-UQCRFS1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102150

Cat. No. GTX102150

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all UQCRFS1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

Mouse brain , Raji

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.32 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human UQCRFS1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952521

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 , RIP1 , RIS1 , RISP , UQCR5

Cellular Localization

Mitochondrion inner membrane

Background

Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis. The transit peptide of the Rieske protein seems to form part of the bc1 complex and is considered to be the subunit 11/IX of that complex.

Database

Research Area

DATA IMAGES

Anti-UQCRFS1 antibody [N1C3] used in Western Blot (WB). GTX102150

GTX102150 WB Image

Sample(30 ug whole cell lysate)
A: Raji(GTX27908)
12% SDS PAGE
GTX102150 diluted at 1:500

Anti-UQCRFS1 antibody [N1C3] used in Western Blot (WB). GTX102150

GTX102150 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
12% SDS PAGE
GTX102150 diluted at 1:1000

Anti-UQCRFS1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102150

GTX102150 ICC/IF Image

UQCRFS1 antibody [N1C3] detects UQCRFS1 protein at mitochondria by immunofluorescent analysis.
Sample: A549 cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min.
Green: UQCRFS1 protein stained by UQCRFS1 antibody [N1C3] (GTX102150) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-UQCRFS1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102150

GTX102150 ICC/IF Image

UQCRFS1 antibody [N1C3] detects UQCRFS1 protein at mitochondria by immunofluorescent analysis.
Sample: H1299 cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min.
Green: UQCRFS1 protein stained by UQCRFS1 antibody [N1C3] (GTX102150) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

Application Reference

Fabienne Wagner et al. bioRxiv 2019; (Epub) Armadillo repeat-containing protein 1 is a dual localization protein associated with mitochondrial intermembrane space bridging complex.
Application : WB
Reactivity : Human
Ishiyama A et al. Neurol Genet 2017; 3 (5) : e184 IBA57 mutations abrogate iron-sulfur cluster assembly leading to cavitating leukoencephalopathy.
Application : WB
Reactivity : Human
Christine Kornelia Ott et al. 0 2015; Diverse Aspects of the Sorting and Assembly Machinery in Human Mitochondria
Application : WB
Reactivity : Human
Gueugneau M et al. BMC Genomics 2014; 15 (1) : 1165 Proteomics of muscle chronological ageing in post-menopausal women.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169