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USP7 antibody

KO/KD
Anti-USP7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125894
Anti-USP7 antibody used in IHC (Paraffin sections) (IHC-P). GTX125894
Anti-USP7 antibody used in IHC (Paraffin sections) (IHC-P). GTX125894
Anti-USP7 antibody used in Immunoprecipitation (IP). GTX125894
Anti-USP7 antibody used in Western Blot (WB). GTX125894
Anti-USP7 antibody used in IHC (Paraffin sections) (IHC-P). GTX125894
Anti-USP7 antibody used in Western Blot (WB). GTX125894
Anti-USP7 antibody used in Western Blot (WB). GTX125894
Anti-USP7 antibody used in IHC (Paraffin sections) (IHC-P). GTX125894
Anti-USP7 antibody used in IHC (Paraffin sections) (IHC-P). GTX125894
Anti-USP7 antibody used in Western Blot (WB). GTX125894
Anti-USP7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125894
Anti-USP7 antibody used in Western Blot (WB). GTX125894
Anti-USP7 antibody used in Western Blot (WB). GTX125894
Anti-USP7 antibody used in Western Blot and Immunoprecipitation (WB IP). GTX125894

Cat. No. GTX125894

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all USP7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

128 kDa. ( Note )

Positive Control

NIH-3T3 , JC , BCL-1 , PC-12 , 293T , A431 , HeLa , HepG2 , USP7 transfected 293T

Product Note

Knockdown/Knockout validation was supported by references (PMID:36878903).

Predict Reactivity

Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.94 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human USP7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885611

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ubiquitin specific peptidase 7 , HAUSP , TEF1

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES

Anti-USP7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125894

GTX125894 ICC/IF Image

USP7 antibody detects USP7 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: USP7 stained by USP7 antibody (GTX125894) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Anti-USP7 antibody used in IHC (Paraffin sections) (IHC-P). GTX125894

GTX125894 IHC-P Image

USP7 antibody detects USP7 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
USP7 stained by USP7 antibody (GTX125894) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-USP7 antibody used in IHC (Paraffin sections) (IHC-P). GTX125894

GTX125894 IHC-P Image

USP7 antibody detects USP7 protein at cytosol and nucleus on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
USP7 antibody (GTX125894) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-USP7 antibody used in Immunoprecipitation (IP). GTX125894

GTX125894 IP Image

Immunoprecipitation of USP7 protein from HepG2 whole cell extracts using 5 μg of USP7 antibody (GTX125894).
Western blot analysis was performed using USP7 antibody (GTX125894).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-USP7 antibody used in Western Blot (WB). GTX125894

GTX125894 WB Image

Sample (30 μg of whole cell lysate)
A: 293T
B: A431
C: HeLa
D: HepG2
5% SDS PAGE
GTX125894 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-USP7 antibody used in IHC (Paraffin sections) (IHC-P). GTX125894

GTX125894 IHC-P Image

USP7 antibody detects USP7 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
USP7 stained by USP7 antibody (GTX125894) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-USP7 antibody used in Western Blot (WB). GTX125894

GTX125894 WB Image

Sample (30 μg of whole cell lysate)
A: PC-12
5% SDS PAGE
GTX125894 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-USP7 antibody used in Western Blot (WB). GTX125894

GTX125894 WB Image

Sample (30 μg of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
5% SDS PAGE
GTX125894 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-USP7 antibody used in IHC (Paraffin sections) (IHC-P). GTX125894

GTX125894 IHC-P Image

USP7 antibody detects USP7 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
USP7 stained by USP7 antibody (GTX125894) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-USP7 antibody used in IHC (Paraffin sections) (IHC-P). GTX125894

GTX125894 IHC-P Image

USP7 antibody detects USP7 protein at cytosol and nucleus on mouse hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hind brain.
USP7 antibody (GTX125894) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-USP7 antibody used in Western Blot (WB). GTX125894

GTX125894 WB Image

Sample (30 μg of whole cell lysate)
A:  Non-transfected 293T lysates 
B:  USP7 transfected 293T lysates
7.5% SDS PAGE
GTX125894 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-USP7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125894

GTX125894 ICC/IF Image

USP7 antibody detects USP7 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: USP7 protein stained by USP7 antibody (GTX125894) diluted at 1:500.
Blue: Hoechst 33343 staining.
Anti-USP7 antibody used in Western Blot (WB). GTX125894

GTX125894 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with USP7 antibody (GTX125894) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-USP7 antibody used in Western Blot (WB). GTX125894

GTX125894 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with USP7 antibody (GTX125894) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-USP7 antibody used in Western Blot and Immunoprecipitation (WB IP). GTX125894

GTX125894 WB IP Image

The data was published in the journal Nat Commun in 2019. PMID: 31519875

REFERENCE

Application Reference

Lee SH et al. PLoS One 2023; 18 (10) : e0290371 USP7 attenuates endoplasmic reticulum stress-induced apoptotic cell death through deubiquitination and stabilization of FBXO7.
Wang N et al. Cell Death Dis 2023; 14 (3) : 182 USP7- and PRMT5-dependent G3BP2 stabilization drives de novo lipogenesis and tumorigenesis of HNSC.
Application : WB
Reactivity : Human
Verwey N et al. Nucleic Acid Ther 2019; (Epub) Antisense-Mediated Skipping of Dysferlin Exons in Control and Dysferlinopathy Patient-Derived Cells.
Application : WB
Ji L et al. Nat Commun 2019; 10 (1) : 4184 USP7 inhibits Wnt/β-catenin signaling through promoting stabilization of Axin.
Application : IP
Reactivity : Human
Vishnoi M et al. Cancer Res 2018; (Epub) Targeting USP7 identifies a metastasis-competent state within bone marrow-resident melanoma CTCs.
Application : ICC/IF
Reactivity : Mouse
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169