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VAPB antibody

KO/KD Orthogonal
Anti-VAPB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131631
Anti-VAPB antibody used in Western Blot (WB). GTX131631
Anti-VAPB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131631
Anti-VAPB antibody used in Western Blot (WB). GTX131631

Cat. No. GTX131631

2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all VAPB products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , HepG2 membrane extract

Predict Reactivity

Mouse, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.47 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human VAPB. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886511

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

VAMP associated protein B and C , ALS8 , VAMP-B , VAP-B

Cellular Localization

Cell membrane; Single-pass type IV membrane protein , Endomembrane system; Single-pass type IV membrane protein

Background

The protein encoded by this gene is a type IV membrane protein found in plasma and intracellular vesicle membranes. The encoded protein is found as a homodimer and as a heterodimer with VAPA. This protein also can interact with VAMP1 and VAMP2 and may be involved in vesicle trafficking. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-VAPB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131631

GTX131631 ICC/IF Image

VAPB antibody detects VAPB protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: VAPB stained by VAPB antibody (GTX131631) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-VAPB antibody used in Western Blot (WB). GTX131631

GTX131631 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with VAPB antibody (GTX131631) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VAPB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131631

GTX131631 ICC/IF Image

VAPB antibody detects VAPB protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: VAPB stained by VAPB antibody (GTX131631) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar= 10μm.

Anti-VAPB antibody used in Western Blot (WB). GTX131631

GTX131631 WB Image

Orthogonal

HepG2 whole cell and membrane extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with VAPB antibody (GTX131631) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

There are currently no references for VAPB antibody (GTX131631). Be the first to share your publications with this product.
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REVIEW

VAPB antibody Cat. No. GTX131631

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Immunoprecipitation(IP)
( Average 5 based on 1 users reviews)
Anti-VAPB antibody used in Immunoprecipitation (IP). GTX131631
Date : Anonymous submitted on 26-Jul-2022
Score :
Application Tested : IP
Sample Species : Hu
Sample : HeLa cells
Primary Antibody : 1:2000, °C, Hr
Lane Description :
SM: 10% starting material
UB: 10% unbound fraction
IP: IP antibody
Figure Note :
1.0μg of antibody to 500μl of Pierce IP Lysis Buffer, together with 30μl of protein A- Sepharose beads.
One ml aliquots at 1.0 mg/ml of lysate were incubated with an antibody-bead conjugate for ~2 hrs at 4ºC.
IB antiobody: 1:2000
Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Anti-VAPB antibody used in Western Blot (WB). GTX131631
Date : Anonymous submitted on 26-Jul-2022
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa WT and VAPB KO cells
Amount used : 50μg
Blocking : 5% milk, 1 hr
Primary Antibody : 1:1000 in 5% BSA and TBST, 4°C, > 12Hr
Figure Note : Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169