VGAT antibody [HL1615] detects VGAT protein at cell membrane by immunohistochemical analysis.
Sample: Frozen-sectioned mouse cerebellum.
Green: VGAT stained by VGAT antibody [HL1615] (GTX637106) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

Boiled and unboiled mouse tissue extract (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with VGAT antibody [HL1615] (GTX637106) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with VGAT antibody [HL1615] (GTX637106) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

VGAT antibody [HL1615] detects VGAT protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
VGAT stained by VGAT antibody [HL1615] (GTX637106) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min