Non-transfected (–) and transfected (+) unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with VGAT antibody [HL1615] (GTX637107) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

VGAT antibody [HL1616] detects VGAT protein at cell membrane by immunohistochemical analysis.
Sample: Frozen-sectioned mouse cerebellum.
Green: VGAT stained by VGAT antibody [HL1616] (GTX637107) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

VGAT antibody [HL1616] detects VGAT protein at nucleus and cytoskeleton by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: VGAT stained by VGAT antibody [HL1616] (GTX637107) diluted at 1:250.
Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

VGAT antibody [HL1616] detects VGAT protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse cerebellum.
Green: VGAT stained by VGAT antibody [HL1616] (GTX637107) diluted at 1:100.

VGAT antibody [HL1616] detects VGAT protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Green: VGAT stained by VGAT antibody [HL1616] (GTX637107) diluted at 1:100.
Red: NF-H, a Cytoplasm marker, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

VGAT antibody [HL1616] detects VGAT protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: VGAT stained by VGAT antibody [HL1616] (GTX637107) diluted at 1:100.
Red: beta Tubulin 3/ Tuj1, a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Boiled and unboiled mouse tissue extract (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with VGAT antibody [HL1616] (GTX637107) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) Boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with VGAT antibody [HL1616] (GTX637107) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

VGAT antibody [HL1616] detects VGAT protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
VGAT stained by VGAT antibody [HL1616] (GTX637107) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min