Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with VIP antibody (GTX129461) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

VIP antibody detects VIP protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse thalamus.
Green: VIP stained by VIP antibody (GTX129461) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

VIP antibody detects VIP protein by western blot analysis. Whole cell extracts (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with VIP antibody (GTX129461) at a dilution of 1:1000.

VIP antibody detects VIP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat pancreas.
VIP stained by VIP antibody (GTX129461) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

VIP antibody detects VIP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
VIP stained by VIP antibody (GTX129461) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min