Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VISA antibody (GTX132521) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

VISA antibody detects VISA protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
VISA stained by VISA antibody (GTX132521) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min