Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with VPS29 antibody [C1C3] (GTX104768) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.