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VPS34 antibody [HL2305]

Recombinant antibody KO/KD Overexpression
Anti-VPS34 antibody [HL2305] used in Western Blot (WB). GTX638372
Anti-VPS34 antibody [HL2305] used in Western Blot (WB). GTX638372
Anti-VPS34 antibody [HL2305] used in Western Blot (WB). GTX638372
Anti-VPS34 antibody [HL2305] used in IHC (Paraffin sections) (IHC-P). GTX638372
Anti-VPS34 antibody [HL2305] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638372
Anti-VPS34 antibody [HL2305] used in Western Blot (WB). GTX638372
Anti-VPS34 antibody [HL2305] used in Western Blot (WB). GTX638372

Cat. No. GTX638372

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2305

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Dog
Package
100 μl ($399),
25 μl ($169)
See all VPS34 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

102 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragmemt of human VPS34.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphatidylinositol 3-kinase catalytic subunit type 3 , VPS34 , Vps34 , hVps34

Cellular Localization

Midbody

Background

Enables 1-phosphatidylinositol-3-kinase activity. Involved in early endosome to late endosome transport and regulation of cytokinesis. Acts upstream of or within autophagy and protein lipidation. Located in autolysosome; late endosome; and midbody. [provided by Alliance of Genome Resources, Apr 2022]

Database

Research Area

DATA IMAGES

Anti-VPS34 antibody [HL2305] used in Western Blot (WB). GTX638372

GTX638372 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS34 antibody [HL2305] (GTX638372) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VPS34 antibody [HL2305] used in Western Blot (WB). GTX638372

GTX638372 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS34 antibody [HL2305] (GTX638372) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VPS34 antibody [HL2305] used in Western Blot (WB). GTX638372

GTX638372 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS34 antibody [HL2305] (GTX638372) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VPS34 antibody [HL2305] used in IHC (Paraffin sections) (IHC-P). GTX638372

GTX638372 IHC-P Image

VPS34 antibody [HL2305] detects VPS34 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
VPS34 stained by VPS34 antibody [HL2305] (GTX638372) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-VPS34 antibody [HL2305] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638372

GTX638372 ICC/IF Image

VPS34 antibody [HL2305] detects VPS34 protein at endoplasmic reticulum and cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: VPS34 stained by VPS34 antibody [HL2305] (GTX638372) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-VPS34 antibody [HL2305] used in Western Blot (WB). GTX638372

GTX638372 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS34 antibody [HL2305] (GTX638372) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-VPS34 antibody [HL2305] used in Western Blot (WB). GTX638372

GTX638372 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS34 antibody [HL2305] (GTX638372) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations