Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (GTX635821) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (GTX635821) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

VPS35 antibody detects VPS35 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: VPS35 stained by VPS35 antibody (GTX635821) diluted at 1:500.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (GTX635821) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (GTX635821) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

VPS35 antibody [HL1017] detects VPS35 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
VPS35 stained by VPS35 antibody [HL1017] (GTX635821) diluted at 1:50.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

VPS35 antibody [HL1017] detects VPS35 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissue.
VPS35 stained by VPS35 antibody [HL1017] (GTX635821) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

VPS35 antibody [HL1017] detects VPS35 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
VPS35 stained by VPS35 antibody [HL1017] (GTX635821) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Whole zebrafish extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (GTX635821) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (GTX635821) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.