vasa antibody detects vasa protein at primordial germ cell on whole-mount zebrafish embryos by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed zebrafish embryos.
vasa antibody (GTX128306) dilution: 1:200.

Vasa antibody detects Vasa protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 1 day-post-fertilization zebrafish embryo.
Green: Vasa stained by Vasa antibody (GTX128306) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

Whole zebrafish extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Vasa antibody (GTX128306) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Vasa antibody detects Vasa protein by western blot analysis.
A. 30 μg Zebrafish 10 hpf lysate/extract
7.5% SDS-PAGE
Vasa antibody (GTX128306) dilution: 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Vasa antibody detects Vasa protein on Medaka by whole mount immunohistochemical analysis.
Sample: 7 days-post-fertilization medaka embryo.
Vasa antibody (GTX128306) dilution: 1:100.

The data was published in the journal Sci Rep in 2017.PMID: 28211534

The data was published in the journal PLoS Genet in 2018.PMID: 30399145