Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Venezuelan Equine Encephalitis Virus nsP1 antibody [HL1472] (GTX636946) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescent analysis of Venezuelan equine encephalitis virus infected cells using Venezuelan Equine Encephalitis Virus nsP1 antibody [HL1472] (GTX636946).
Sample: Multiple virus infected cells slide.
Green: Venezuelan Equine Encephalitis Virus nsP1 antibody [HL1472] (GTX636946) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

Venezuelan equine encephalitis virus nsp1 antibody [HL1472] detects Venezuelan equine encephalitis virus nsp1 protein at cytoplasm by immunohistochemical analysis.
Sample:Paraffin-embedded mock and Venezuelan equine encephalitis virus nsp1 transfected 293T cell pellet.
Green: Venezuelan equine encephalitis virus nsp1 stained by Venezuelan equine encephalitis virus nsp1 antibody [HL1472] (GTX636946) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Venezuelan equine encephalitis virus nsp1 antibody [HL1472] detects Venezuelan equine encephalitis virus nsp1 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Venezuelan equine encephalitis virus nsp1 stained by Venezuelan equine encephalitis virus nsp1 antibody [HL1472] (GTX636946) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).