Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Venezuelan Equine Encephalitis Virus nsP3 antibody [HL1502] (GTX636976) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Venezuelan Equine Encephalitis Virus nsP3 antibody [HL1502] (GTX636976) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescent analysis of Venezuelan equine encephalitis virus infected cells using Venezuelan Equine Encephalitis Virus nsP3 antibody [HL1502] (GTX636976).
Sample: Multiple virus infected cells slide.
Green: Venezuelan Equine Encephalitis Virus nsP3 antibody [HL1502] (GTX636976) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

Venezuelan equine encephalitis virus nsp3 antibody [HL1502] detects Venezuelan equine encephalitis virus nsp3 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mock and VEEV nsp3 (Venezuelan equine encephalitis virus) - transfected 293T cell pellet.
Green: Venezuelan equine encephalitis virus nsp3 stained by Venezuelan equine encephalitis virus nsp3 antibody [HL1502] (GTX636976) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Venezuelan equine encephalitis virus nsp3 antibody [HL1502] detects Venezuelan equine encephalitis virus nsp3 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Venezuelan equine encephalitis virus nsp3 stained by Venezuelan equine encephalitis virus nsp3 antibody [HL1502] (GTX636976) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).