Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT7812] (GTX629743) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Wild-type (WT) and Vimentin knockout (KO) 293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT7812] (GTX629743) diluted at 1:20000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with Vimentin antibody [GT7812] (GTX629743) diluted at 1:5000 and competitor's antibody diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT7812] (GTX629743) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT7812] (GTX629743) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Vimentin antibody [GT7812] detects Vimentin protein at fibroblasts on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Vimentin antibody [GT7812] (GTX629743) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Vimentin antibody [GT7812] detects Vimentin protein at cytoplasm in human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung carcinoma.
Vimentin antibody [GT7812] (GTX629743) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Vimentin antibody [GT7812] detects Vimentin protein by western blot analysis.
A. 30 μg C8D30 whole cell lysate/extract
B. 30 μg NIH-3T3 whole cell lysate/extract
C. 30 μg C2C12 whole cell lysate/extract
10% SDS-PAGE
Vimentin antibody [GT7812] (GTX629743) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Sandwich ELISA detection of recombinant full-length Vimentin protein using GTX629743 as capture antibody at concentration of 5 μg/mL and GTX100619 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Vimentin antibody [GT7812] detects Vimentin proteins in embryonic mouse brain by immunohistochemical analysis.
Sample: Frozen section of embryonic mouse brain (mE18.5).
Red: Vimentin antibody [GT7812] (GTX629743) diluted at 1:1000.
Blue: DAPI.

Sandwich ELISA detection of recombinant full-length Vimentin protein using GTX629743 as capture antibody at concentration of 5 μg/mL and GTX112661 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Vimentin antibody [GT7812] detects Vimentin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Vimentin stained by Vimentin antibody [GT7812] (GTX629743) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Vimentin antibody [GT7812] detects Vimentin protein at fibroblasts on human endometrial carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
Vimentin antibody [GT7812] (GTX629743) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT7812] (GTX629743) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT7812] (GTX629743) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Vimentin antibody [GT7812] detects Vimentin protein by immunohistochemical analysis.
Sample: Paraffin-embedded dog spleen.
Vimentin stained by Vimentin antibody [GT7812] (GTX629743) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Vimentin antibody [GT7812] detects Vimentin protein by immunohistochemical analysis.
Sample: Paraffin-embedded dog pancreas.
Vimentin stained by Vimentin antibody [GT7812] (GTX629743) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Vimentin antibody [GT7812] detects Vimentin protein at cytoskeleton by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Vimentin stained by Vimentin antibody [GT7812] (GTX629743) diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Vimentin antibody [GT7812] detects Vimentin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog colon.
Vimentin stained by Vimentin antibody [GT7812] (GTX629743) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Vimentin antibody [GT7812] (GTX629743) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Vimentin antibody [GT7812] detects Vimentin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog intestine.
Vimentin stained by Vimentin antibody [GT7812] (GTX629743) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min