Summary
Vimentin antibody detects vimentin, a type III intermediate filament protein with a predicted molecular weight of 54 kDa. Vimentin is highly expressed in cells of mesodermal origin as well as in some ectodermal cells, and is often used as a marker of mesenchymal stem cells. It is also utilized in combination with E-cadherin to study the morphological and phenotypic changes in cells undergoing epithelial–mesenchymal transition during development and tumor metastasis. In addition, vimentin antibody is a valuable reagent to stain neuronal precursors and developing radial glia cells.
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:5000-1:50000 |
1:100-1:1000 |
1:100-1:1000 |
Assay dependent |
1:100-1:500 |
Assay dependent |
Assay dependent |
Assay dependent |
Assay dependent |
Note :
Sandwich ELISA
Capture : GTX629743 / GTX629744, Detection : GTX100619
|
Not tested in other applications.
Calculated MW
Observed MW
56 kDa. The observed M.W. is based on the following publication. PMID: 23185433 and 24926698
Positive Control
293T
Predict Reactivity
Bovine, Chicken, Guinea pig, Xenopus tropicalis, Chimpanzee(>80% identity)
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.43 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Vimentin. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1952557
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.