Vinculin antibody [HL1873] detects Vinculin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
Vinculin stained by Vinculin antibody [HL1873] (GTX637622) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Vinculin antibody [HL1873] (GTX637622) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Vinculin antibody [HL1873] (GTX637622) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole zebrafish extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Vinculin antibody [HL1873] (GTX637622) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Vinculin antibody [HL1873] (GTX637622) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Vinculin antibody [HL1873] (GTX637622) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.