Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with WAVE1 antibody (GTX133263) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with WAVE1 antibody (GTX133263) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with WAVE1 antibody (GTX133263) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

WAVE1 antibody detects WAVE1 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: WAVE1 stained by WAVE1 antibody (GTX133263) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

WAVE1 antibody detects WAVE1 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
WAVE1 antibody (GTX133263) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

WAVE1 antibody detects WAVE1 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
WAVE1 antibody (GTX133263) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

WAVE1 antibody detects WAVE1 protein at cytoplasm by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: WAVE1 protein stained by WAVE1 antibody (GTX133263) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.