Immunofluorescence analysis of methanol-fixed HeLa, using WNK1(GTX106197) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using WNK1(GTX106197) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

WNK1 antibody detects WNK1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
WNK1 stained by WNK1 antibody (GTX106197) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

WNK1 antibody detects WNK1 protein at cytoplasm in human lung adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung adenocarcinoma.
WNK1 antibody (GTX106197) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with WNK1 antibody (GTX106197) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with WNK1 antibody (GTX106197) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.