GeneTex

  • Country / Location Selection

United States (US)

WNK1 antibody

Orthogonal
Anti-WNK1 antibody used in Western Blot (WB). GTX106197
Anti-WNK1 antibody used in Western Blot (WB). GTX106197
Anti-WNK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX106197
Anti-WNK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX106197
Anti-WNK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX106197
Anti-WNK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106197

Cat. No. GTX106197

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all WNK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

251 kDa. ( Note )

Predict Reactivity

Rat, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.18 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human WNK1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2038244

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

WNK lysine deficient protein kinase 1 , HSAN2 , HSN2 , KDP , PPP1R167 , PRKWNK1 , PSK , p65

Cellular Localization

Cytoplasm

Background

The WNK1 gene encodes a cytoplasmic serine-threonine kinase expressed in distal nephron.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-WNK1 antibody used in Western Blot (WB). GTX106197

GTX106197 WB Image

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with WNK1 antibody (GTX106197) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-WNK1 antibody used in Western Blot (WB). GTX106197

GTX106197 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with WNK1 antibody (GTX106197) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-WNK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX106197

GTX106197 IHC-P Image

WNK1 antibody detects WNK1 protein at cytoplasm in human lung adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung adenocarcinoma.
WNK1 antibody (GTX106197) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-WNK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX106197

GTX106197 IHC-P Image

WNK1 antibody detects WNK1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
WNK1 stained by WNK1 antibody (GTX106197) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-WNK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX106197

GTX106197 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using WNK1(GTX106197) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-WNK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106197

GTX106197 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using WNK1(GTX106197) antibody at 1:200 dilution.

REFERENCE

Application Reference

Ho YJ et al. In Vivo 2020; 34 (5) : 2631-2640 Prognostic and Clinical Implications of WNK Lysine Deficient Protein Kinase 1 Expression in Patients With Hepatocellular Carcinoma.
Application : IHC-P
Reactivity : Human
Ding L et al. JCI Insight 2017; 2 (22) Akt3 inhibits adipogenesis and protects from diet-induced obesity via WNK1/SGK1 signaling
Application : WB
Reactivity : Mouse
Liu Y et al. J Am Soc Nephrol 2015; 26 (4) : 844-54 WNK1 activates large-conductance Ca2+-activated K+ channels through modulation of ERK1/2 signaling.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for WNK1 antibody (GTX106197). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169