Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with WRN antibody [HL1729] (GTX637377) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

WRN antibody [HL1729] detects WRN protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
WRN stained by WRN antibody [HL1729] (GTX637377) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min