Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt1 antibody (GTX111231) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Wnt1 antibody detects Wnt1 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of human WNT1-transfected 293T cells
10% SDS PAGE
Wnt1 antibody (GTX111231) dilution: 1:1000