APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:1000-1:10000 |
Assay dependent |
Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
This antibody is specific for human Wnt3a protein, and it does not cross react with human Wnt1, Wnt2, Wnt2b, Wnt3, Wnt4, Wnt5a, Wnt6, Wnt7a, Wnt7b, Wnt8a, Wnt8b, Wnt9a, Wnt9b, Wnt10a, Wnt10b, and Wnt16 protein.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment of human Wnt3a.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Wnt family member 3A
Cellular Localization
Secreted, extracellular space, extracellular matrix,Secreted
Background
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product. This gene is clustered with WNT14 gene, another family member, in chromosome 1q42 region. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX637660 IHC-P Image
Wnt3a antibody [HL1911] detects secreted Wnt3a protein by immunohistochemical analysis.Sample: Paraffin-embedded human prostate cancer.Wnt3a stained by Wnt3a antibody [HL1911] (GTX637660) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637660 IHC-P Image
Wnt3a antibody [HL1911] detects Wnt3a protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.Wnt3a stained by Wnt3a antibody [HL1911] (GTX637660) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637660 ICC/IF Image
Wnt3a antibody [HL1911] detects Wnt3a protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.Green: Wnt3a stained by Wnt3a antibody [HL1911] (GTX637660) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).Scale bar= 10μm.
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GTX637660 WB Image
Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt3a antibody [HL1911] (GTX637660) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637660 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt3a antibody [HL1911] (GTX637660) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637660 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt3a antibody [HL1911] (GTX637660) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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REFERENCE
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REVIEW
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