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Wnt4 antibody [HL1889]

Recombinant antibody Overexpression
Anti-Wnt4 antibody [HL1889] used in Western Blot (WB). GTX637638
Anti-Wnt4 antibody [HL1889] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637638
Anti-Wnt4 antibody [HL1889] used in IHC (Paraffin sections) (IHC-P). GTX637638
Anti-Wnt4 antibody [HL1889] used in Western Blot (WB). GTX637638
Anti-Wnt4 antibody [HL1889] used in Western Blot (WB). GTX637638

Cat. No. GTX637638

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1889

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all Wnt4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Product Note

This antibody is specific for human Wnt4 protein, and it does not cross react with human Wnt1, Wnt2, Wnt2b, Wnt3, Wnt3a, Wnt5a, Wnt6, Wnt7a, Wnt7b, Wnt8a, Wnt8b, Wnt9a, Wnt9b, Wnt10a, Wnt10b, and Wnt16 protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human Wnt4.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Wnt family member 4 , SERKAL , WNT-4

Cellular Localization

Secreted, extracellular space, extracellular matrix

Background

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family, and is the first signaling molecule shown to influence the sex-determination cascade. It encodes a protein which shows 98% amino acid identity to the Wnt4 protein of mouse and rat. This gene and a nuclear receptor known to antagonize the testis-determining factor play a concerted role in both the control of female development and the prevention of testes formation. This gene and another two family members, WNT2 and WNT7B, may be associated with abnormal proliferation in breast tissue. Mutations in this gene can result in Rokitansky-Kuster-Hauser syndrome and in SERKAL syndrome. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Wnt4 antibody [HL1889] used in Western Blot (WB). GTX637638

GTX637638 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt4 antibody [HL1889] (GTX637638) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Wnt4 antibody [HL1889] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637638

GTX637638 ICC/IF Image

Wnt4 antibody [HL1889] detects Wnt4 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Wnt4 stained by Wnt4 antibody [HL1889] (GTX637638) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Wnt4 antibody [HL1889] used in IHC (Paraffin sections) (IHC-P). GTX637638

GTX637638 IHC-P Image

Wnt4 antibody [HL1889] detects Wnt4 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Wnt4 stained by Wnt4 antibody [HL1889] (GTX637638) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Wnt4 antibody [HL1889] used in Western Blot (WB). GTX637638

GTX637638 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt4 antibody [HL1889] (GTX637638) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Wnt4 antibody [HL1889] used in Western Blot (WB). GTX637638

GTX637638 WB Image

Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt4 antibody [HL1889] (GTX637638) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations