Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt4 antibody [HL1889] (GTX637638) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Wnt4 antibody [HL1889] detects Wnt4 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Wnt4 stained by Wnt4 antibody [HL1889] (GTX637638) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt4 antibody [HL1889] (GTX637638) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt4 antibody [HL1889] (GTX637638) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.