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Wnt9a antibody [HL2422]

Recombinant antibody Orthogonal Overexpression
Anti-Wnt9a antibody [HL2422] used in IHC (Paraffin sections) (IHC-P). GTX638644
Anti-Wnt9a antibody [HL2422] used in IHC (Paraffin sections) (IHC-P). GTX638644
Anti-Wnt9a antibody [HL2422] used in Western Blot (WB). GTX638644
Anti-Wnt9a antibody [HL2422] used in Western Blot (WB). GTX638644
Anti-Wnt9a antibody [HL2422] used in Western Blot (WB). GTX638644

Cat. No. GTX638644

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2422

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Wnt9a products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide of human Wnt9a.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Wnt family member 9A , WNT14

Cellular Localization

Secreted, extracellular space, extracellular matrix,Secreted

Background

The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is expressed in gastric cancer cell lines. The protein encoded by this gene shows 75% amino acid identity to chicken Wnt14, which has been shown to play a central role in initiating synovial joint formation in the chick limb. This gene is clustered with another family member, WNT3A, in the chromosome 1q42 region. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Wnt9a antibody [HL2422] used in IHC (Paraffin sections) (IHC-P). GTX638644

GTX638644 IHC-P Image

Wnt9a antibody [HL2422] detects secreted Wnt9a protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
Wnt9a stained by Wnt9a antibody [HL2422] (GTX638644) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Wnt9a antibody [HL2422] used in IHC (Paraffin sections) (IHC-P). GTX638644

GTX638644 IHC-P Image

Wnt9a antibody [HL2422] detects secreted Wnt9a protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat intestine.
Wnt9a stained by Wnt9a antibody [HL2422] (GTX638644) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Wnt9a antibody [HL2422] used in Western Blot (WB). GTX638644

GTX638644 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt9a antibody [HL2422] (GTX638644) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Wnt9a antibody [HL2422] used in Western Blot (WB). GTX638644

GTX638644 WB Image

Orthogonal

Various whole cell extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt9a antibody [HL2422] (GTX638644) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Wnt9a antibody [HL2422] used in Western Blot (WB). GTX638644

GTX638644 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt9a antibody [HL2422] (GTX638644) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations