XRCC4 antibody

Cat. No. GTX109632
References 9
Cat. No.  GTX109632
Orthogonal
Anti-XRCC4 antibody used in IHC (Paraffin sections) (IHC-P). GTX109632
GTX109632 IHC-P Image

XRCC4 antibody detects XRCC4 protein at nucleus on HBL435 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HBL435 xenograft.
XRCC4 antibody (GTX109632) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

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Anti-XRCC4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109632
GTX109632 ICC/IF Image

XRCC4 antibody detects XRCC4 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: XRCC4 stained by XRCC4 antibody (GTX109632) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

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Anti-XRCC4 antibody used in Western Blot (WB). GTX109632
GTX109632 WB Image
Orthogonal

HeLa whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with XRCC4 antibody (GTX109632) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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Anti-XRCC4 antibody used in Western Blot (WB). GTX109632
GTX109632 WB Image
Orthogonal

The data was published in the journal PLoS One in 2013. PMID: 23405159

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Anti-XRCC4 antibody used in IHC (Paraffin sections) (IHC-P). GTX109632
Anti-XRCC4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109632
Anti-XRCC4 antibody used in Western Blot (WB). GTX109632
Anti-XRCC4 antibody used in Western Blot (WB). GTX109632
  • Host
    Rabbit
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Applications
    WB ICC/IF IHC-P
  • Reactivity
    Human, Mouse
Summary
XRCC4 antibody detects X-ray repair cross-complementing protein 4 (XRCC4; predicted molecular weight ~38 kDa), a crucial component of the canonical non-homologous end-joining pathway to repair DNA double-strand breaks. The protein functions by stabilizing and activating DNA Ligase IV, as well as bridging the DNA ends. Mutations or disruption of XRCC4 have been linked to a large number of cancers as well as to a form of dwarfism.
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