Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with YAP1 antibody (GTX129151) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with YAP1 antibody (GTX129151) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

YAP1 antibody detects YAP1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human glioblastoma.
YAP1 stained by YAP1 antibody (GTX129151) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with YAP1 antibody (GTX129151) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

YAP1 antibody detects YAP1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: YAP1 protein stained by YAP1 antibody (GTX129151) diluted at 1:1000.
Blue: Hoechst 33342 staining.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with YAP1 antibody (GTX129151) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

YAP1 antibody detects YAP1 protein at nucleus on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
YAP1 antibody (GTX129151) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

YAP1 antibody immunoprecipitates YAP1 protein in IP experiments.
IP samples: HeLa whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of YAP1 protein by 4 μg YAP1 antibody (GTX129151)
7.5 % SDS-PAGE
The immunoprecipitated YAP1 protein was detected by YAP1 antibody (GTX129151) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

The data was published in the journal Nat Commun in 2019. PMID: 31477715

The data was published in the journal Nat Commun in 2019. PMID: 31477715