Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with YY1 antibody (GTX110625) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

YY1 antibody detects YY1 protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
10 % SDS-PAGE
YY1 antibody (GTX110625) dilution: 1:1000

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using YY1(GTX110625) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunohistochemical analysis of paraffin-embedded 59T xenograft, using YY1(GTX110625) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 ug of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX110625 diluted at 1:2000

YY1 antibody detects YY1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: YY1 stained by YY1 antibody (GTX110625) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar= 10 μm.