Non-transfected (–) and transfected (+) 293T whole cell extracts (60 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Yellow Fever Virus NS2B Protein antibody (GTX134028) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.