Yellow  fever virus viral lysate (0.1 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Yellow Fever virus prM Protein antibody (GTX133957) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Yellow Fever Virus prM Protein antibody (GTX133957) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.