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ZC3H12A antibody [HL1442]

Recombinant antibody KO/KD Orthogonal Overexpression
Anti-ZC3H12A antibody [HL1442] used in Western Blot (WB). GTX636914
Anti-ZC3H12A antibody [HL1442] used in Western Blot (WB). GTX636914
Anti-ZC3H12A antibody [HL1442] used in Western Blot (WB). GTX636914
Anti-ZC3H12A antibody [HL1442] used in Western Blot (WB). GTX636914

Cat. No. GTX636914

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1442

Isotype

 IgG

Application

 WB

Reactivity

 Human, Cat
Package
100 μl ($399),
25 μl ($169)
See all ZC3H12A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ZC3H12A. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

zinc finger CCCH-type containing 12A , MCPIP , MCPIP-1 , MCPIP1 , Reg1 , dJ423B22.1

Cellular Localization

Nucleus,Cytoplasm

Background

ZC3H12A is an MCP1 (CCL2; MIM 158105)-induced protein that acts as a transcriptional activator and causes cell death of cardiomyocytes, possibly via induction of genes associated with apoptosis.[supplied by OMIM, Mar 2008]

Database

Research Area

DATA IMAGES

Anti-ZC3H12A antibody [HL1442] used in Western Blot (WB). GTX636914

GTX636914 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZC3H12A antibody [HL1442] (GTX636914) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ZC3H12A antibody [HL1442] used in Western Blot (WB). GTX636914

GTX636914 WB Image

Orthogonal

Untreated (–) and treated (+) THP-1 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZC3H12A antibody [HL1442] (GTX636914) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ZC3H12A antibody [HL1442] used in Western Blot (WB). GTX636914

GTX636914 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ZC3H12A antibody [HL1442] (GTX636914) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ZC3H12A antibody [HL1442] used in Western Blot (WB). GTX636914

GTX636914 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 | 20 | 20 | 10 | 10 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZC3H12A antibody [HL1442] (GTX636914) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations