Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZC3HAV1 antibody [N3C2], Internal (GTX120134) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ZC3HAV1 antibody [N3C2], Internal detects ZC3HAV1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZC3HAV1 stained by ZC3HAV1 antibody [N3C2], Internal (GTX120134) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

ZC3HAV1 antibody [N3C2], Internal detects ZC3HAV1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
ZC3HAV1 stained by ZC3HAV1 antibody [N3C2], Internal (GTX120134) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

ZC3HAV1 antibody [N3C2], Internal detects ZC3HAV1 protein at cytoplasm in human lung cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
ZC3HAV1 antibody [N3C2], Internal (GTX120134) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

The data was published in the 2018 in PLoS Pathog. PMID: 30016363