Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using ZCCHC4(GTX119809) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunofluorescence analysis of paraformaldehyde-fixed A431, using ZCCHC4(GTX119809) antibody at 1:500 dilution.

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ZCCHC4 antibody [N1N3] (GTX119809) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ZCCHC4 antibody [N1N3] (GTX119809) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.