Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ZKSCAN3 antibody [N1C2] (GTX106039) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ZNF306 (GTX106039) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded human normal lung, using ZKSCAN3(GTX106039) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min