Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZNF649 antibody (GTX121655) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data are based on Human Protein Atlas program.

Immunohistochemical analysis of paraffin-embedded U373 xenograft, using ZNF649(GTX121655) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min