Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [C3], C-term (GTX108587) diluted at 1:500.

ZO-1 antibody detects ZO-1 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
5 % SDS-PAGE
ZO-1 antibody (GTX108587) dilution: 1:500

Sample (30 ug of whole cell lysate)
A: HCT116
5% SDS PAGE
GTX108587 diluted at 1:1000