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ZO-1 antibody

KO/KD Orthogonal
Anti-ZO-1 antibody used in Western Blot (WB). GTX108592
Anti-ZO-1 antibody used in Western Blot (WB). GTX108592
Anti-ZO-1 antibody used in IHC (Paraffin sections) (IHC-P). GTX108592
Anti-ZO-1 antibody used in IHC (Paraffin sections) (IHC-P). GTX108592
Anti-ZO-1 antibody used in Western Blot (WB). GTX108592
Anti-ZO-1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108592
Anti-ZO-1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108592
Anti-ZO-1 antibody used in Western Blot (WB). GTX108592
Anti-ZO-1 antibody used in Western Blot (WB). GTX108592
Anti-ZO-1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX108592

Cat. No. GTX108592

Reference 18
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Bovine, Dog, Pig, Monkey
Package
100 μl ($399),
25 μl ($169)
See all ZO-1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IHC-Fr Assay dependent

Note :

WB
The use of GTX108592 for mouse is based on IHC-P testing. For WB using mouse samples, we recommend GTX108613
Not tested in other applications.

Calculated MW

195 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.19 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ZO-1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952260

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tight junction protein 1 , ZO-1

Cellular Localization

Cell membrane; Peripheral membrane protein; Cytoplasmic side , Cell junction , tight junction

Background

This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ZO-1 antibody used in Western Blot (WB). GTX108592

GTX108592 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody (GTX108592) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ZO-1 antibody used in Western Blot (WB). GTX108592

GTX108592 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody (GTX108592) diluted at 1:1000.

Anti-ZO-1 antibody used in IHC (Paraffin sections) (IHC-P). GTX108592

GTX108592 IHC-P Image

ZO-1 antibody detects ZO-1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
ZO-1 stained by ZO-1 antibody (GTX108592) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ZO-1 antibody used in IHC (Paraffin sections) (IHC-P). GTX108592

GTX108592 IHC-P Image

ZO-1 antibody detects ZO-1 protein at cell membrane and cytoplasm in mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
ZO-1 antibody (GTX108592) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ZO-1 antibody used in Western Blot (WB). GTX108592

GTX108592 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody (GTX108592) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ZO-1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108592

GTX108592 ICC/IF Image

ZO-1 antibody detects ZO-1 protein at cell membrane and cytoplasm by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZO-1 stained by ZO-1 antibody (GTX108592) diluted at 1:1000.

Anti-ZO-1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108592

GTX108592 ICC/IF Image

ZO-1 antibody detects ZO-1 protein at cell membrane and cytoplasm by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZO-1 stained by ZO-1 antibody (GTX108592) diluted at 1:1000.

Anti-ZO-1 antibody used in Western Blot (WB). GTX108592

GTX108592 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody (GTX108592) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ZO-1 antibody used in Western Blot (WB). GTX108592

GTX108592 WB Image

Orthogonal

The data was published in the journal Oncotarget in 2016. PMID: 26517522

Anti-ZO-1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX108592

GTX108592 IHC-Fr Image

The data was published in the journal Proc Natl Acad Sci U S A in 2019. PMID: 31064871

REFERENCE

Application Reference

Chua HH et al. Cell Mol Gastroenterol Hepatol 2024; 17 (3) : 361-381 Antagonism Between Gut Ruminococcus gnavus and Akkermansia muciniphila Modulates the Progression of Chronic Hepatitis B.
Han P et al. Cell Commun Signal 2022; 20 (1) : 149 Cell adhesion molecule BVES functions as a suppressor of tumor cells extrusion in hepatocellular carcinoma metastasis.
Application : WB, ICC/IF
Reactivity : Human
Wei MF et al. Oxid Med Cell Longev 2022; 2022 : 7957255 Atorvastatin Attenuates Radiotherapy-Induced Intestinal Damage through Activation of Autophagy and Antioxidant Effects.
Application : WB, IHC-P
Reactivity : Mouse
Peng-Hsiang Fang et al. Mol Med 2022; 28 (1) : 71 Cobalt protoporphyrin promotes human keratinocyte migration under hyperglycemic conditions.
Application : WB
Reactivity : Human
Tomi E.D. et al. Int J Mol Sci. 2022; 23 (3) : 1647 Upregulation of miR-34a-5p, miR-20a-3p and miR-29a-3p by Onconase in A375 Melanoma Cells Correlates with the Downregulation of Specific Onco-Proteins.
Application : WB
Reactivity : Human
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Submit a Reference

REVIEW

ZO-1 antibody Cat. No. GTX108592

Rating
( Average 5 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Anti-ZO-1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108592
Date : Anonymous submitted on 25-Oct-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : Caco-2 cells
Blocking : 0.5% skim milk in TBST, RT, 1Hr
Fixation : 4%PFA/PBS, 15 min
Permeabilization /Antigen retrieval : TBST
Primary Antibody : 1:2000, 25°C, 12Hr
Submit a Review
Application Reference
Chua HH et al. Cell Mol Gastroenterol Hepatol 2024; 17 (3):361-381 Antagonism Between Gut Ruminococcus gnavus and Akkermansia muciniphila Modulates the Progression of Chronic Hepatitis B.
Han P et al. Cell Commun Signal 2022; 20 (1):149 Cell adhesion molecule BVES functions as a suppressor of tumor cells extrusion in hepatocellular carcinoma metastasis.
Application : WB, ICC/IF
Reactivity : Human
Wei MF et al. Oxid Med Cell Longev 2022; 2022:7957255 Atorvastatin Attenuates Radiotherapy-Induced Intestinal Damage through Activation of Autophagy and Antioxidant Effects.
Application : WB, IHC-P
Reactivity : Mouse
Peng-Hsiang Fang et al. Mol Med 2022; 28 (1):71 Cobalt protoporphyrin promotes human keratinocyte migration under hyperglycemic conditions.
Application : WB
Reactivity : Human
Tomi E.D. et al. Int J Mol Sci. 2022; 23 (3):1647 Upregulation of miR-34a-5p, miR-20a-3p and miR-29a-3p by Onconase in A375 Melanoma Cells Correlates with the Downregulation of Specific Onco-Proteins.
Application : WB
Reactivity : Human
Wenyi Fang et al. Mol Med Rep 2021; 23 (3):1 Effects of Qing Hua Chang Yin on lipopolysaccharide?induced intestinal epithelial tight junction injury in Caco?2 cells.
Application : WB
Reactivity : Human
Yoshinobu Kariya et al. Commun Biol 2021; 4 (1):490 αvβ3 Integrin induces partial EMT independent of TGF-β signaling.
Application : WB, ICC/IF
Reactivity : Human
Watanabe D et al. Pharmaceutics 2021; 13 (9) Characterization of a Primate Blood-Brain Barrier Co-Culture Model Prepared from Primary Brain Endothelial Cells, Pericytes and Astrocytes.
Application : WB
Reactivity : Monkey
Barbian ME et al. Pediatr Res 2021; (Epub) Butyrate supplementation to pregnant mice elicits cytoprotection against colonic injury in the offspring.
Application : IHC-P
Minamide K et al. Sci Rep 2020; 10 (1):14639 IRF2 maintains the stemness of colonic stem cells by limiting physiological stress from interferon.
Application : IHC-P
Reactivity : Mouse
Bisi S et al. Nat Commun 2020; 11 (1):3516 IRSp53 controls plasma membrane shape and polarized transport at the nascent lumen in epithelial tubules.
Xie MY et al. Biochem Pharmacol 2020; 175:113898 Porcine milk exosome miRNAs protect intestinal epithelial cells against deoxynivalenol-induced damage.
Application : WB
Reactivity : Pig
Kaliannan K et al. Commun Biol 2019; 2:276 Multi-omic analysis in transgenic mice implicates omega-6/omega-3 fatty acid imbalance as a risk factor for chronic disease.
Application : IHC-P
Reactivity : Mouse
Koch F et al. Proc Natl Acad Sci U S A 2019; 116 (21):10333-10338 Heat stress directly impairs gut integrity and recruits distinct immune cell populations into the bovine intestine.
Application : IHC-Fr
Reactivity : Bovine
Li W et al. J Clin Invest 2017; (Epub) The FOXN3-NEAT1-SIN3A repressor complex promotes progression of hormonally responsive breast cancer.
Application : WB
Reactivity : Human
Jo H et al. Food Chem Toxicol 2017; 108 (Pt A):203-213 Oxyresveratrol improves tight junction integrity through the PKC and MAPK signaling pathways in Caco-2 cells.
Application : WB
Reactivity : Human
Sheng-Huei Yang et al. Oncotarget. 2016; 7 (1) Integrin β3 and LKB1 are independently involved in the inhibition of proliferation by lovastatin in human intrahepatic cholangiocarcinoma
Application : WB
Reactivity : Human
Hwang D et al. Biomed Pharmacother 2016; Conditioned medium from LS 174T goblet cells treated with oxyresveratrol strengthens tight junctions in Caco-2 cells.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169