Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody (GTX108592) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody (GTX108592) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody (GTX108592) diluted at 1:1000.

ZO-1 antibody detects ZO-1 protein at cell membrane and cytoplasm in mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
ZO-1 antibody (GTX108592) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

ZO-1 antibody detects ZO-1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
ZO-1 stained by ZO-1 antibody (GTX108592) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody (GTX108592) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ZO-1 antibody detects ZO-1 protein at cell membrane and cytoplasm by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZO-1 stained by ZO-1 antibody (GTX108592) diluted at 1:1000.

ZO-1 antibody detects ZO-1 protein at cell membrane and cytoplasm by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ZO-1 stained by ZO-1 antibody (GTX108592) diluted at 1:1000.

The data was published in the journal Oncotarget in 2016. PMID: 26517522

The data was published in the journal Proc Natl Acad Sci U S A in 2019. PMID: 31064871