Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Zika virus Envelope protein antibody [GT363] (GTX634155) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634155 as capture antibody at concentration of 5 μg/mL and GTX133325 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Immunofluorescent analysis of non-infected and infected vero cells using Zika virus Envelope protein antibody [GT363] (GTX634155).
Green: Zika virus Envelope protein antibody [GT363] (GTX634155) diluted at 1:500.

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX133326 as capture antibody at concentration of 5 μg/mL and GTX634155 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634155 as capture antibody at concentration of 5 μg/mL and GTX133326 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Immunofluorescent analysis of arboviruses infected cells using Zika virus Envelope protein antibody [GT363] (GTX634155).
Sample: Mock and zika virus-infected cells.
Green: Zika virus Envelope protein antibody [GT363] (GTX634155) diluted at 1:100.

Sandwich ELISA detection of Zika virus-infected lysate using GTX634155 as capture antibody at concentration of 5 μg/mL and GTX133326 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

The data was published in the journal Sci Rep in 2017. PMID: 29093521