Sample (30 ug of whole cell lysate)
A: NIH-3T3
7.5% SDS PAGE
GTX111167 diluted at 1:3000

alpha Actinin 2 antibody [N2C1], Internal detects alpha Actinin 2 protein at cytoplasm and membrane on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
alpha Actinin 2 antibody [N2C1], Internal (GTX111167) diluted at 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using actinin, alpha 2(GTX111167) antibody at 1:200 dilution.

alpha Actinin 2 antibody detects alpha Actinin 2 protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 2 antibody (GTX111167) diluted at 1:5000.

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: H1299
7.5% SDS PAGE
GTX111167 diluted at 1:1000

Sample (10 μg of whole cell lysate)
A: adult zebrafish
7.5% SDS PAGE
GTX111167 diluted at 1:3000

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using alpha Actinin 2 antibody [N2C1], Internal (GTX111167) at 1:300 dilution.

Mouse tissue extract (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 2 antibody [N2C1], Internal (GTX111167) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.