Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
7.5% SDS PAGE
GTX103216 diluted at 1:10000

Immunoprecipitation of alpha Actinin 3 protein from HeLa whole cell extracts using 5 μg of alpha Actinin 3 antibody (GTX103216).
Western blot analysis was performed using alpha Actinin 3 antibody (GTX103216).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

alpha Actinin 3 antibody detects alpha Actinin 3 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 3 antibody (GTX103216) diluted by 1:10000.

Sample (30 ug of whole cell lysate)
A: PC-12
7.5% SDS PAGE
GTX103216 diluted at 1:10000

Sample (30 μg of whole cell lysate)
A: whole zebrafish
7.5% SDS PAGE
GTX103216 diluted at 1:1000